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The Journal of Horticultural Science & Biotechnology

Vol. 81 No: 5

Title:
Genetic diversity in fruiting-mei, apricot, plum and peach revealed by AFLP analysis

Authors:
J. FANG, J. TAO and C.T. CHAO

pp: 898-902

Abstract:
High-throughput amplified fragment length polymorphism (AFLP) analysis, employing a two-dye automated DNA sequencer, was used to genotype accessions of four important Prunus spp. fruit crops in China, fruiting-mei (P. mume), apricot (P. armeniaca), plum (P. salicina, and P. ussuriensis) and peach (P. persica). Nine primer pairs generated a total of 2,089 AFLP bands, of which 94.4% were polymorphic. Levels of polymorphism and the genetic relationships among the crops were studied.The fixation index (FST) of the four different fruit trees ranged from 0.53 - 0.82, and the average genetic diversity (AGD) in these species ranged from 3.2% to 12.8%.These four stone fruit species could be clustered into four clear groups on a phylogenetic tree based on bootstrap analysis. Phylogenetically, fruiting-mei was shown to be closest to apricot and most distant from peach.

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