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The Journal of Horticultural Science & Biotechnology
Vol. 81 No: 6
Title:
Metabolic responses to salt stress in cell suspension cultures of sensitive and resistant Citrus
Authors:
A.L. FERREIRA and M.E. LIMA-COSTA
pp: 983-988
Abstract:
The acquisition of resistance to salt stress was investigated in cell suspension cultures of two Citrus cultivars, Citrus hybrid cv. ´Carvalhal` and Citrus sinensis cv. ´Valencia late`. The metabolic responses in terms of scavenging enzyme activities (e.g., superoxide dismutase, catalase and guaiacol peroxidase), partitioning of Na+, Cl- and K+ ions, and proline accumulation were investigated in relation to growth and salt damage at the cellular level. These two cell lines use different metabolic and cell biochemical strategies to tolerate salt stress. The ´Carvalhal` cell line displayed a salt resistant behaviour, even at high salt concentrations. This salt-resistance behaviour operated primarily by impeding the uptake of Na+ and Cl- ions combined with intracellular proline accumulation and a high level of scavenging of reactive oxygen species (ROS). On the other hand, the cv. ´Valencia late` appeared to be salt-sensitive. Proline accumulation can be used as an indicator of the salt stress imposed on Citrus cell lines under the conditions used here.
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