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The Journal of Horticultural Science & Biotechnology

Vol. 82 No: 2

Title:
Determination and confirmation of S-RNase genotypes of apple pollinators and cultivars

Authors:
S. MATSUMOTO, T. EGUCHI, H. BESSHO and K. ABE

pp: 323-329

Abstract:
We analysed the S-RNase genotypes of 23 crab apple (Malus spp.) pollinators and 102 cultivars of domestic apple (Malus pumila Mill.) by PCR amplification and digestion.Within the 23 pollinators, four pollinators, ´Hopa`, ´Jack`, ´Pink Perfection` and ´Profusion B`, each had two unidentified S-RNase alleles. These cultivars should be useful pollinators for all domestic cultivars. Twenty-one of the domestic cultivars exhibited S-genotypes contrary to those expected from their supposed parentage, suggesting that one or both reported parents were wrong. We confirmed many of the S-RNase genotypes by pollination tests.

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