Vol. 82 No: 5

Title:
Bioreactor culture and plant regeneration from cell clusters of the aromatic plant, Lavandula angustifolia 'Munstead'

Authors:
XIAOJUN WANG, LIANG JIN, MEI LI, MINAN ZHAO, HAIQING ZHAO and YAOZU XU

pp: 781-785

Abstract:
An efficient protocol is outlined for the establishment of proliferating cell cluster cultures using a bioreactor, and subsequent regeneration of the aromatic plant, Lavandula angustifolia ‘Munstead’. Red calli were obtained by culturing leaflet explants on basal MS medium supplemented with 0.23 μM 2,4-dichlorophenoxyacetic (2,4-D) and 2.22 μM 6-benzylaminopurine (BA), and subsequent sub-culturing. Calli were then transferred to basal MS liquid medium containing 0.23 μM 2,4-D and 2.22 μM BA to initiate suspension cultures. Cell clusters were obtained by sieving the initial suspensions and sub-culturing them in basal MS liquid medium supplemented with 0.23 μM 2,4-D plus 2.22 μM BA and 1.89 μM abscisic acid, by means of shake culture, or bioreactor culture. After 20 d of culture, cell clusters were transferred to basal MS medium containing 1.14 μM indole-3-acetic acid and produced a high number of regenerated plants, which were successfully transferred to soil.

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