Vol. 83 No: 3

Title:
Using Allium alien monosomic addition lines to study chlorophyll degradation in stored Japanese bunching onion

Authors:
P.K. DISSANAYAKE, S. YAGUCHI, N. YAMAUCHI and M. SHIGYO

pp: 388-394

Abstract:
Chlorophyll (Chl) degradation and the formation of Chl derivatives in stored leaves of Japanese bunching onion (JBO; Allium fistulosum L., genome FF) were determined to elucidate the mechanism of Chl degradation by using alien monosomic addition lines (AMALs; FF+1A to FF+8A) of JBO from shallot (A. cepa L. Aggregatum group, genome AA).Year-round changes in Chl content in JBO and in the AMALs were also detected over 2 years.The Chl content in FF was significantly influenced by the alien chromosomes from shallot. In particular, the Chl contents of FF+4A or FF+5A, were higher in Winter than in FF, which suggests that chromosomes 4A and 5A from shallot had a larger influence on the formation of Chl than the other chromosomes. Chl contents in FF+3A and FF+5A decreased greatly in the leaves of AMALs and JBO during storage at 25°C, whereas in FF+4A the Chl content was the lowest compared to the control, FF. Chlorophyllide (Chlide) a, C13²-hydroxychlorophyll (OHChl) a, pheophorbide a, and pheophytin (Phy) a, as derivatives of Chl a, were present mainly during storage of JBO and the AMALs. Phy a levels increased with Chl degradation, especially in FF+3A, while OHChl a levels in FF and FF+3A showed a decline during storage. In addition, Chlide a levels in all lines diminished during storage.Thus, Chl a may be degraded, in part, through Phy a, as well as Chlide a and OHChl a, in JBO and the AMALs during storage.

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