Vol. 83 No: 4

Title:
Regeneration and molecular characterisation of two interspecific somatic hybrids of Citrus for potential rootstock improvement

Authors:
W.W. GUO, R.C. WU, Y.J. CHENG and X.X. DENG

pp: 407-410

Abstract:
To produce allotetraploid somatic hybrid citrus rootstocks, protoplasts isolated from ‘Dancy’ tangerine or ‘Kinnow’ mandarin (Citrus reticulata Blanco) were electrically fused with mesophyll protoplasts of ‘Goutou’ sour orange (C. aurantium L.). Three and six plants were regenerated from these two fusions, respectively. They were confirmed to be tetraploids and diploids by flow cytometry. Nuclear DNA simple sequence repeats (SSR) analysis verified that they were true hybrids, with novel bands present. Chloroplast DNA SSR analysis showed random inheritance of chloroplast DNA (cpDNA) in the ‘Dancy’ + ‘Goutou’ hybrids, while all the cpDNA of the ‘Kinnow’ + ‘Goutou’ hybrids was derived from ‘Kinnow’, the callus parent. Cleaved amplified polymorphic sequence (CAPS) analysis using mitochondria-specific DNA (mtDNA) primer pairs, followed by restriction endonuclease digestion, showed that all the mtDNA of the ‘Dancy’ + ‘Goutou’ somatic hybrids was from ‘Dancy’, the callus parent; while that of the ‘Kinnow’ + ‘Goutou’ diploid hybrids was not distinguishable using various primer/endonuclease combinations. The potential of these two somatic hybrids for the improvement of Citrus rootstocks is discussed.

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