Vol. 83 No: 6

Title:
Genetic and epigenetic analyses of in vitro-grown plants of Arachis villosulicarpa Hoehne (Leguminosae) obtained from seed explants through different regeneration pathways

Authors:
G. PACHECO, S.R.S. CARDOSO, R.F. GAGLIARDI, J.F.M. VALLS, P.C.G. FERREIRA, M.A. CARDOSO and E. MANSUR

pp: 737-742

Abstract:
In vitro conservation techniques are considered suitable strategies for ex situ conservation of wild species of Arachis. However, there is a potential risk of genetic and epigenetic alterations induced by tissue culture conditions. The goal of this work was to determine the influence of explant type and regeneration pathway on sequence modifications and changes in the DNA methylation status of in vitro-grown plants of A. villosulicarpa using Amplified Fragment Length Polymorphism (AFLP) and Methylation-Sensitive Amplified Polymorphism (MSAP) analyses. Regeneration from embryo axes occurred through multiplication of pre-existing meristems. Cotyledons and embryonic leaflets displayed direct and indirect organogenesis, respectively. No polymorphic AFLP amplification fragments were detected among the regenerants. Conversely, MSAP analysis showed a decrease in the levels of DNA methylation in cotyledon- and embryonic leaflet-derived plants.

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