Vol. 83 No: 6

Title:
Identification of S-genotypes and novel S-RNase alleles in Prunus mume

Authors:
W. HENG, H.Q. WU, Q.X. CHEN, J. WU, S.X. HUANG and S.L. ZHANG

pp: 689-694

Abstract:
Prunus mume (Japanese apricot) cultivars are self-incompatible and orchards require pollinator cultivars to guarantee optimal fruit production. Identification of the self-incompatibility (S) genotypes of P. mume cultivars is therefore useful for choosing pollinators and for breeding. To elucidate the S-genotypes of eleven Chinese and one introduced cultivar, PCR was performed with primers amplifying the variable second intron of the S-RNase gene. The S-genotyping results were as follows: S₄S₁₂ for ‘Dantaofen’; S₃S₄ for ‘Duoezhusha’; S₁₂S₁₃ for ‘Fubantiaozhi’; S₁₅S₁₆ for ‘Hongding’; S₁₃S₁₄ for ‘Longyanmei’; S₅S₁₁ for ‘Musashino’; S₁₀S₁₄ for ‘Xiaoyezhugan’; and S₃S₁₅ for ‘Qingfeng’, ‘Qingjia’, ‘Xiyeqing’, ‘Dayeqing’ and ‘Changnong 17’. We identified seven new S-RNase alleles, S₁₀-RNase to S₁₆-RNase, deposited in GenBank under the accession numbers: DQ011150, DQ201191, DQ201192, DQ345781, DQ768219, DQ903312 and EF990750, respectively.

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