Vol. 84 No: 2

Title:
Molecular tagging and mapping of a major linked marker for taproot thickness in a Brassica crop

Authors:
J.S. CAO, G. LU, L.J. JIANG, L. CHEN, X. XIANG and H. CHEN

pp: 175-180

Abstract:
A DNA marker linked to the locus controlling storage-root thickness was identified using bulked segregant analysis (BSA) in an F₂ population derived from a cross between turnip (Brassica rapa L. ssp. rapifera Matzg.) and rapeseed (Brassica rapa L.). Root-thickness in the F₂ plants showed a continuous distribution from 2.2 – 10.7 cm, suggesting the effects of a few major and minor genes. BSA, using three bulks based on root-thickness, suggested that an AFLP marker (E_ACCM_CAG-220) which appeared only in plants with a thickened root, was linked to a locus that controlled root size. This AFLP band was cloned, sequenced, and successfully converted into a simple, dominant, sequence characterised amplified region (SCAR) marker. This marker may help to identify turnip genotypes with thicker taproots in segreating progenies of root crops during marker-assisted selection (MAS) breeding programmes.

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