Vol. 84 No: 3

Title:
Metabolite profiling by capillary electrophoresis-mass spectrometry reveals aberrant putrescine accumulation associated with idiopathic symptoms of gentian plants

Authors:
H. TAKAHASHI, I. MUNEMURA, T. NAKATSUKA, M. NISHIHARA and H. UCHIMIYA

pp: 312-318

Abstract:
The disease, yellow dwarf-like symptom (YDS), in gentians has the characteristics of leaf etiolation, gall formation, and shortening of the stem internodes. This disease leads to plant death, and has thus become a serious problem in commercial gentian production. Despite intensive pathological investigation, the aetiology of the disease remains unclear. To increase our knowledge about YDS, we conducted metabolome analysis using capillary electrophoresis-mass spectrometry. Quantification of the metabolites in YDS-affected tissues showed that YDS particularly affected arginine-derived metabolites, including arginine, ornithine, citrulline, and putrescine. The concentration of putrescine increased 83-fold, and was accompanied by increased expression of the arginine decarboxylase gene. Thus putrescine synthesis and its accumulation appear to be the key metabolic changes associated with YDS in gentians.

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