Vol. 85 No: 2

Title:
Structural and functional analyses of two new S-RNase alleles, S_si5 and S_ad5, in apple

Authors:
S. MATSUMOTO, K. YAMADA, K. SHIRATAKE, K. OKADA and K. ABE

pp: 131-136

Abstract:
We determined the genomic DNA sequences of two new S-RNase alleles, S_si5 and S_ad5, in apple. Both consisted of 232 deduced amino acids, and contained five conserved domains (C1, C2, C3, RC4, and C5), and an intron specific to S-RNase genes of the Rosaceae, at the appropriate positions.We developed a PCR-RFLP method to identify these alleles, and clarified their occurrence in several Malus species, such as M. orientalis (W1-11-13; ID number 90104; S_si5), M. sieversii (W1-10-49; ID number 90100; S_si5), and M. sylvestris (ID number 88076; S_ad5) and cultivars, such as ‘Adersleber Calville’ (S_ad5) and ‘Cox’s Pomona’ (S_ad5). Cross-pollination tests confirmed that S_si5 and S_ad5 were functionally distinct in spite of their identical rosaceous hypervariable region (RHV), which appears to be critical for S-allele discrimination in the Rosaceae. We recommend use of the next available numbers, S₃₃ and S₃₄, instead of S_si5 and S_ad5, respectively, since the allele annotations are only transitory. This is the first known case in apple in which two S-RNase alleles containing an identical RHV act as different alleles.

Full text: JHSB Subscribers     ISHS members & other users
(PDF 132120 bytes)

Translate:

Go back to previous page