Vol. 86 No: 5

Title:
Development of new microsatellite markers from an enriched genomic library of date palm (Phoenix dactylifera L.)

Authors:
S.A. MIRBABAEE, M. MARDI, P. MAHMOODI, S.M. PIRSEYEDI, A. ABBASI, M. FARSI, H. SOLEIMANI, G. BAKHSHIKHANIKI, S. MOHAJERI-NARAGHI, M. ZEINOLABEDINI and S.M. KHAYAM-NEKOUEI

pp: 539-541

Abstract:
Date palm (Phoenix dactylifera L) has been cultivated since early times. The high nutritional, economic, and social values of date palm culture are well known. In this study, we report on the isolation and characterisation of nine novel polymorphic nuclear microsatellite loci from a repeat-enriched genomic library of P. dactylifera L. The genetic diversity of these loci was estimated in 50 accessions of P. dactylifera L. The number of alleles per locus ranged from two-to-four, with an average of 2.5 alleles per locus. The observed (H_o) and expected (H_e) heterozygosities ranged from 0.11 – 1.00 and from 0.10 – 0.74, respectively. These microsatellite markers could be useful for studies on the genetic diversity and conservation genetics of date palm.

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