The Journal of Horticultural Science & Biotechnology
Vol. 87 No: 1
Rapid in vitro multiplication of Sida cordifolia L. ¿ a threatened medicinal plant
V.P. PRAMOD and M. JAYARAJ
An efficient, reproducible protocol has been developed for in vitro multiplication of Sida cordifolia L. High-frequency, multiple shoots (90%) were obtained indirectly from nodal explants.
Callus was induced when nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5 mg l-1 Kinetin (Kn). These nodal calli were then cultured in order to differentiate multiple shoots on MS medium supplemented with 0.5 mg l-1 Kn plus 0.5 mg l-1 naphthaleneacetic acid (NAA). Roots were induced from these multiple shoots following culture on MS medium supplemented with 0.8 mg l-1 NAA for 4 weeks.
Finally, these in vitro plantlets were hardened, acclimatised, and successfully transferred to the field.
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