Vol. 87 No: 2

Title:
Molecular mapping of the downy mildew resistance gene Ppa3 in cauliflower (Brassica oleracea var. botrytis L.)

Authors:
S. SINGH, S.R. SHARMA, P. KALIA, R. DESHMUKH, V. KUMAR, P. SHARMA and T.R. SHARMA

pp: 137-143

Abstract:
The present study aimed to construct a linkage map for the downy mildew resistance gene, Ppa3, in cauliflower. Ppa3 is a dominant, single locus gene in cauliflower which confers downy mildew resistance. Out of 190 markers screened, 13 were found to be polymorphic between the two parental lines, ‘BR-2’ and ‘Pusa Himjyoti’. Among these 13 polymorphic markers, six were random amplified polymorphic DNA (RAPD) markers (OPB18₁₂₇₆, OPB20₁₁₇₄, OPC14₁₁₈₆, OPC1₈₅₀, OPF1₉₆₀, and OPE14₁₈₈₁) and the remaining seven were inter-simple sequence repeat (ISSR) markers (ISSR5₅₂₅, ISSR8₁₁₂₅, ISSR11₅₅₀, ISSR12₁₀₅₀, ISSR23₁₁₀₃, ISSR30₅₇₅, and ISSR35₆₂₀). Furthermore, bulked segregant analysis identified only three markers (OPC14₁₁₈₆, OPE14₁₈₈₁, and ISSR23₁₁₀₃) as being polymorphic. An F₂ mapping population consisting of 120 plants was analysed using the latter three polymorphic markers and a linkage map was constructed for Ppa3. OPC14₁₁₈₆, was located 22.3 cM from the Ppa3 gene on the top-arm end, with OPE14₁₈₈₁ and ISSR23₁₁₀₃, at 10.6 cM and 26.4 cM, respectively on the other side of the Ppa3 gene on the linkage map. This information may be useful for further studies to identify more closely-linked markers for the Ppa3 gene.

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